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73 Digital PCR (dPCR) Revolution: Transforming Precision Diagnostics and Research Introduction
Digital PCR (dPCR) is an ultra‐sensitive molecular method for absolute quantification of nucleic acids based on dividing a sample into numerous tiny reactions (droplets, wells, or microreactors), amplifying PCR, and counting positive over negative partitions. Unlike the conventional quantitative PCR (qPCR) based on amplification curves and standards, dPCR provides direct counts of...
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